.- Nuria Almunia Santiago (Supervisors: Manuel Avilés Sánchez and Emilio Gómez Sánchez)

.- Pilar Soria Monzó (Supervisors: Manuel Avilés Sánchez and María José Izquierdo Rico)

.- Inmaculada Torres González (Supervisors: Manuel Avilés Sánchez and Aníbal Nieto)

2023

54.- Adenylate kinase 9 is essential for sperm function and male fertility in mammals. O'Callaghan E, Navarrete-Lopez P, Štiavnická M, Sánchez JM, Maroto M, Pericuesta E, Fernández-González R, O'Meara C, Eivers B, Kelleher MM, Evans RD, Mapel XM, Lloret-Villas A, Pausch H, Balastegui-Alarcón M, Avilés M, Sanchez-Rodriguez A, Roldan ERS, McDonald M, Kenny DA, Fair S, Gutiérrez-Adán A, Lonergan P. Proc Natl Acad Sci U S A. 2023 Oct 17;120(42):e2305712120. doi: 10.1073/pnas.2305712120.

53.- Colostrum Features of Active and Recovered COVID-19 Patients Revealed Using Next-Generation Proteomics Technique, SWATH-MS. Hernández-Caravaca I, Moros-Nicolás C, González-Brusi L, Romero de Ávila MJ, De paco Matallan C, Pelegrín P, Castaño-Molina MA, Díaz-Meca L, Sáncherz-Romero J, Martínez-Alarcón L, Avilés M, Izquierdo-Rico MJ. Children 2023, 10, 1423. https://doi.org/10.3390/children10081423

52.- Boar sperm motility is modulated by CCK at a low concentration of bicarbonate under capacitation conditions. Moros-Nicolás C, López-Úbeda R, Luongo C, Sòria-Monzó P, Abril-Sánchez S, Grudzinska P, Avilés M, Izquierdo-Rico MJ, García-Vázquez FA. Reprod Domest Anim. 2023 Jun 18. doi: 10.1111/rda.14412. Online ahead of print. PMID: 37332097. https://onlinelibrary.wiley.com/doi/epdf/10.1111/rda.14412

51.- Human Sperm Head Vacuoles Are Related to Nuclear-Envelope Invaginations. Gómez-Torres MJ, Luna-Romero J, Fernández-Colom PJ, Aizpurua J, Avilés M, Romero A. Int J Mol Sci. 2023 Jun 12;24(12):10027. doi: 10.3390/ijms241210027. PMID: 37373176. https://www.mdpi.com/1422-0067/24/12/10027

2022

50.- Analysis of Minor Proteins Present in Breast Milk by Using WGA Lectin. Hernández-Caravaca I, Cabañas A, López-Úbeda R, González-Brusi L, Guillén-Martínez A, Izquierdo-Rico MJ, Muñoz-Rodríguez MN, Avilés M, Ruiz García MJ. Children (Basel). 2022 Jul 20;9(7):1084. doi: 10.3390/children9071084.

49.- Molecular Chaperone HSPA2 Distribution During Hyaluronic Acid Selection in Human Sperm. María José Gómez-Torres, Natalia Huerta-Retamal, Paula Sáez-Espinosa, Laura Robles-Gómez, Manuel Avilés, Jon Aizpurua. Reprod Sci. 2022 Jul 11. doi: 10.1007/s43032-022-01031-9.

2021

48.- Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Tursiops truncatus). Fuentes-Albero MC, González-Brusi L, Cots P, Luongo C, Abril-Sánchez S, Ros-Santaella JL, Pintus E, Ruiz-Díaz S, Barros-García C, Sánchez-Calabuig MJ, García-Párraga D, Avilés M, Izquierdo Rico MJ, García-Vázquez FA. Front Cell Dev Biol. 2021 Jul 16;9:673961. doi: 10.3389/fcell.2021.673961

47.- Evidence of haptoglobin in the porcine female genital tract during oestrous cycle and its effect on in vitro embryo production. García-Vázquez FA, Moros-Nicolás C, López-Úbeda R, Rodríguez-Tobón E, Guillén-Martínez A, Ross JW, Luongo C, Matás C, Hernández-Caravaca I, Avilés M, Izquierdo-Rico MJ. Sci Rep. 2021 Jun 8;11(1):12041. doi: 10.1038/s41598-021-90810-6. PMID: 34103548, https://www.nature.com/articles/s41598-021-90810-6

46.- Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM). Gómez-Torres MJ, Huerta-Retamal N, Robles-Gómez L, Sáez-Espinosa P, Aizpurua J, Avilés M, Romero A. Cells. 2021 Jan 23;10(2):222. doi: 10.3390/cells10020222. PMID: 33498624

45.- New Insights into the Mammalian Egg Zona Pellucida. Moros-Nicolás C, Chevret P, Jiménez-Movilla M, Algarra B, Cots-Rodríguez P, González-Brusi L, Avilés M, Izquierdo-Rico MJ. Int J Mol Sci. 2021 Mar 23;22(6):3276. doi: 10.3390/ijms22063276. PMID: 33806989 https://www.mdpi.com/1422-0067/22/6/3276

44.- Human sperm chaperone HSPA2 distribution during in vitro capacitation. Huerta-Retamal N, Sáez-Espinosa P, Robles-Gómez L, Avilés M, Romero A, Aizpurua J, Gómez-Torres MJ. J Reprod Immunol. 2021 Feb;143:103246. doi: 10.1016/j.jri.2020.103246. Epub 2020 Nov 12. PMID: 33246276

43.- ZP4 Is Present in Murine Zona Pellucida and Is Not Responsible for the Specific Gamete Interaction. Izquierdo-Rico MJ, Moros-Nicolás C, Pérez-Crespo M, Laguna-Barraza R, Gutiérrez-Adán A, Veyrunes F, Ballesta J, Laudet V, Chevret P, Avilés M. Front Cell Dev Biol. 2021 Jan 18;8:626679. doi: 10.3389/fcell.2020.626679. eCollection 2020. PMID: 33537315 https://www.frontiersin.org/articles/10.3389/fcell.2020.626679/full

2020

42.- Sperm Proteome after Interaction with Reproductive Fluids in Porcine: From the Ejaculation to the Fertilization Site. Luongo C, González-Brusi L, Cots-Rodríguez P, Izquierdo-Rico MJ, Avilés M, García-Vázquez FA. Int J Mol Sci. 2020 Aug 22;21(17):6060. doi: 10.3390/ijms21176060. PMID: 32842715

41.- A Comparative View on the Oviductal Environment during the Periconception Period. González-Brusi L, Algarra B, Moros-Nicolás C, Izquierdo-Rico MJ, Avilés M, Jiménez-Movilla M. Biomolecules. 2020 Dec 17;10(12):1690. doi: 10.3390/biom10121690. PMID: 33348856

40.- Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid. Rodríguez-Alonso B, Maillo V, Acuña OS, López-Úbeda R, Torrecillas A, Simintiras CA, Sturmey R, Avilés M, Lonergan P, Rizos D. Int J Mol Sci. 2020 Feb 29;21(5):1681. doi: 10.3390/ijms21051681. PMID: 32121434 https://www.mdpi.com/1422-0067/21/5/1681

39.- Influence of in vitro capacitation time on structural and functional human sperm parameters. Sáez-Espinosa P, Huerta-Retamal N, Robles-Gómez L, Avilés M, Aizpurua J, Velasco I, Romero A, Gómez-Torres MJ. Asian J Androl. 2020 Sep-Oct;22(5):447-453. doi: 10.4103/aja.aja_104_19. PMID: 31621655

38.- FE-SEM Characterization of α-Mannose Density and Surface Mapping Changes in Human Sperm Head During In Vitro Capacitation. Gómez-Torres MJ, Robles-Gómez L, Huerta-Retamal N, Sáez-Espinosa P, Avilés M, Aizpurua J, Romero A. Microsc Microanal. 2020 Dec;26(6):1220-1225. doi: 10.1017/S1431927620024630. PMID: 33121558

2019

37.- AUTORES (p.o. de firma): Ismael Lamas-Toranzo, Noelia Fonseca Balvís, Ana Querejeta-Fernández, María José Izquierdo-Rico, Leopoldo González-Brusi, Pedro L Lorenzo, Pilar García-Rebollar, Manuel Avilés, Pablo Bermejo-Álvarez. TÍTULO: ZP4 confers structural properties to the zona pellucida essential for embryo development. Ref. Revista: eLife 2019;8:e48904 DOI: 10.7554/eLife.48904. https://elifesciences.org/articles/48904

36.- AUTORES (p.o. de firma): Sáez-Espinosa P, Huerta-Retamal N, Robles-Gómez L, Avilés M, Aizpurua J, Velasco I, Romero A, Gómez-Torres MJ. TÍTULO: Influence of in vitro capacitation time on structural and functional human sperm parameters. Ref. Revista: Asian J Androl. 2019 Oct 15. doi: 10.4103/aja.aja_104_19

2018

35.- AUTORES (p.o. de firma): Coy Fuster, P., Avilés, M., Latorre Reviriego, R. (2018). Fallopian Tube/Oviduct. In M. K. Skinner (Ed.), Encyclopedia of Reproduction. vol. 2, pp. 276–281. Academic Press: Elsevier. http://dx.doi.org/10.1016/B978-0-12-801238-3.64400-8

34.- AUTORES (p.o. de firma): Algarra B, Maillo V, Avilés M, Gutiérrez-Adán A, Rizos D, Jimenez-Movilla M TÍTULO: Effects of recombinant OVGP1 protein on in vitro bovine embryo development. Ref. Revista: J Reprod Dev. 2018 Aug 6. doi: 10.1262/jrd.2018-058. PMID: 30078833

33.- AUTORES (p.o. de firma): Pérez-Cerezales S, Ramos-Ibeas P, Acuña OS, Avilés M, Coy P, Rizos D, Gutiérrez-Adán A. TÍTULO: The oviduct: from sperm selection to the epigenetic landscape of the embryo. Ref. Revista: Biol Reprod. 2017 Dec 8. doi: 10.1093/biolre/iox173.

32.- AUTORES (p.o. de firma): Moros-Nicolás C, Chevret P, Izquierdo-Rico MJ, Holt WV, Esteban-Díaz D, López-Béjar M, Martínez-Nevado E, Nilsson MA, Ballesta J, Avilés M. TÍTULO: Composition of marsupial zona pellucida: a molecular and phylogenetic approach. Ref. Revista: Reprod Fertil Dev. 2017 Nov 22. doi: 10.1071/RD16519.

31.- AUTORES (p.o. de firma): Moros-Nicolás C, Leza A, Chevret P, Guillén-Martínez A, González-Brusi L, Boué F, Lopez-Bejar M, Ballesta J, Avilés M, Izquierdo-Rico MJ. TÍTULO: Analysis of ZP1 gene reveals differences in zona pellucida composition in carnivores. Ref. Revista: Reprod Fertil Dev. 2017 Jul 6. doi: 10.1071/RD17022

30.- AUTORES (p.o. de firma): Acuña OS, Avilés M, López-Úbeda R, Guillén-Martínez A, Soriano-Úbeda C, Torrecillas A, Coy P, Izquierdo-Rico MJ. TÍTULO: Differential gene expression in porcine oviduct during the oestrous cycle. Ref. Revista: Reprod Fertil Dev. 2017 May 19. doi: 10.1071/RD16457.

29.- AUTORES (p.o. de firma): Algarra B, Han L, Avilés M, Coy P, Jovine L, Jiménez-Movilla M. TÍTULO: The C-terminal region of OVGP1 remodels the zona pellucida and modifies fertility parameters. Ref. Revista: Scientific Reports. Sept 7th, 2016. http://www.nature.com/articles/srep32556

28.- AUTORES (p.o. de firma): Defaus S, Avilés M, Andreu D, Gutiérrez-Gallego R. TÍTULO: Identification of bovine sperm surface proteins involved in carbohydrate-mediated fertilization interactions. Ref. Revista: Mol Cell Proteomics. 2016 Apr 19. pii: mcp.M115.057703. http://www.ncbi.nlm.nih.gov/pubmed/27094474

27.- AUTORES (p.o. de firma): Stetson I, Avilés M, Moros C, García-Vázquez FA, Gimeno L, Torrecillas A, Aliaga C, Bernardo-Pisa MV, Ballesta J, Izquierdo-Rico MJ. TÍTULO: Four glycoproteins are expressed in the cat zona pellucida. Ref. Revista: Theriogenology. 2015 Apr 15;83(7):1162-73. doi: 10.1016/j.theriogenology.2014.12.019.

26.- AUTORES (p.o. de firma): Gómez-Torres MJ, García EM, Guerrero J, Medina S, Izquierdo-Rico MJ, Gil-Izquierdo Á, Orduna J, Savirón M, González-Brusi L, Ten J, Bernabeu R, Avilés M. TÍTULO: Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization. Ref. LIBRO: Reprod Biol Endocrinol. 2015 Nov 9;13(1):123. doi: 10.1186/s12958-015-0118-9. http://www.rbej.com/content/13/1/123

25.- AUTORES (p.o. de firma): Avilés M, Izquierdo-Rico MJ, Jiménez-Movilla M TÍTULO: Interacciones espermatozoide-zona pelúcida. Ref. LIBRO: Bases genéticas y moleculares de la fecundación humana. Editores Alberto Romeu y Pedro J. Fernández. Editorial Médica. Madrid. Octubre 2013. Capítulo 8; páginas: 215-253. ISBN: 978-84-616-4788-0.

24.- AUTORES (p.o. de firma): Saavedra MD, Mondéjar I, Coy P, Betancourt M, González-Márquez H, Jiménez-Movilla M, Avilés M, Romar R TÍTULO: Calreticulin from suboolemmal vesicles affects membrane regulation of polyspermy. Ref. REVISTA: Reproduction. 2014 Jan 7. PMID: 24398873

23.- AUTORES (p.o de firma): Mondéjar I, Martínez-Martínez I, Avilés M, Coy P. TÍTULO: Identification of Potential Oviductal Factors Responsible of the Zona Pellucida Hardening and Monospermy During Fertilization in Mammals. Ref. REVISTA/LIBRO: Biology of Reproduction 2013; Jul 17 PMID: 23863406 http://www.biolreprod.org/content/89/3/67.long

22.- AUTORES (p.o de firma): Mondéjar I, Avilés M, Coy P. TÍTULO: The human is an exception to the evolutionarily-conserved phenomenon of pre-fertilization zona pellucida resistance to proteolysis induced by oviductal fluid. Ref. REVISTA/LIBRO: Human Reproduction 2013; 28(3):718-28. doi: 10.1093/humrep/des423

21.- AUTORES (p.o de firma): Coy P, Garcia-Vázquez FA, Visconti PE, Avilés M. TÍTULO: Roles of the oviduct in mammalian fertilization. Ref. REVISTA/LIBRO: Reproduction. 2012 Dec;144(6):649-60. doi: 10.1530/REP-12-0279 http://www.reproduction-online.org/content/144/6/649.full.pdf+html

20.- AUTORES (p.o de firma): Gómez-Torres MJ, Avilés M, Girela JL, Murcia V, Fernández-Colom PJ, Romeu A, De Juan J TITULO: Characterization of the lectin binding pattern in human spermatozoa after swim-up selection. REF. REVISTA/LIBRO: Histology and Histopathology 2012, 27 (12): 1621-1628.

19.- AUTORES (p.o de firma): Stetson I, Izquierdo-Rico MJ, Moros C, Chevret P, Lorenzo PL, Ballesta J, Rebollar PG, Gutiérrez-Gallego R, Avilés M. TITULO: Rabbit zona pellucida composition: A molecular, proteomic and phylogenetic approach. REF. REVISTA/LIBRO: J Proteomics. 2012 Jul 26

18.- AUTORES (p.o de firma): Mondéjar I, Acuña OS, Izquierdo-Rico MJ, Coy P, Avilés M. TITULO: The oviduct: functional genomic and proteomic approach. REF. REVISTA/LIBRO: Reprod Domest Anim. 2012 Jun;47 Suppl 3:22-9. doi: 10.1111/j.1439-0531.2012.02027.x.

17.- AUTORES (p.o de firma): Izquierdo-Rico MJ, Gimeno L, Jiménez-Cervantes C, Ballesta J, Avilés M. TITULO: “Biosynthesis of hamster zona pellucida is restricted to the oocyte” REF. REVISTA/LIBRO: Theriogenology. 2011 Feb;75(3):463-72

16.- AUTORES (p.o de firma): Avilés M, Gutiérrez-Adán A, Coy P TITULO: “Oviductal secretions: will they be key factors for the future ARTs? REF. REVISTA/LIBRO: Molecular Human Reproduction, 2010 Dec;16(12):896-906. http://molehr.oxfordjournals.org/content/16/12/896.full.pdf+html

15.- AUTORES (p.o de firma): Coy P, Avilés M. TITULO: “What controls polyspermy in mammals, the oviduct or the oocyte?” REF. REVISTA/LIBRO: Biol Rev Camb Philos Soc 2010 Aug;85(3):593-605.

14.- AUTORES (p.o. de firma): Canovas S, Romar R, Grullon LA, Avilés M, Coy P. TÍTULO: Pre-fertilization zona pellucida hardening by different cross-linkers affects IVF in pigs and cattle and improves embryo production in pigs. REF. REVISTA/LIBRO: Reproduction. 2009;137(5):803-12. http://www.reproduction-online.org/content/137/5/803.full.pdf+html

13.- AUTORES (p.o. de firma): Canovas S, Romar R, Grullon LA, Avilés M, Coy P. TÍTULO: Pre-fertilization zona pellucida hardening by different cross-linkers affects IVF in pigs and cattle and improves embryo production in pigs. REF. REVISTA/LIBRO: Reproduction. 2009;137(5):803-12.

12.- AUTORES (p.o de firma): Jiménez-Movilla M, Martínez-alonso E, Castells MT, Izquierdo-Rico MJ, Saavedra MD, Gutiérrez-Gallego R, Fayrer-Hosken R, Ballesta J, Avilés M TITULO: Cytochemical and biochemical evidences for a complex tridimensional structure of the hamster zona pellucida. REF. REVISTA/LIBRO: Histology and Histopathology 2009 May;24(5):599-609.

11.- AUTORES (p.o de firma): Coy P, Cánovas S, Romar R, Mondéjar I, Saavedra MD, Grullón L, Matás C and Avilés M TITULO: Oviduct-specific glycoprotein and heparin modulate sperm-zona pellucida interaction during mammalian fertilization REF. REVISTA/LIBRO: Proceedings of the National Academy Sciences USA vol 105: 15809-15814 http://www.pnas.org/content/105/41/15809.full.pdf+html?with-ds=yes

10.- AUTORES (p.o de firma): Carrasco LC, Romar R, Aviles M, Gadea J, Coy P. TITULO: Determination of glycosidase activity in bovine oviductal fluid at the different phases of the estrous cycle. REF. REVISTA/LIBRO: Reproduction Fertility and Development 20:808-817. 2008

9.- AUTORES (p.o de firma): Carrasco LC, Romar R, Avilés M, Gadea J, Coy P TITULO: Determination of glycosidase activity in porcine oviductal fluid at the different phases of the estrous cycle. REF. REVISTA/LIBRO: Reproduction 136(6):833-842. 2008. http://www.reproduction-online.org/content/136/6/833.full.pdf+html

8.- AUTORES (p.o de firma): Coy P, Grullon L, Cánovas S, Romar R, Matas C, Avilés M TITULO: Hardening of the zona pellucida of unfertilized eggs can reduce polyspermic fertilization in the pig and cow. REF. REVISTA/LIBRO: Reproduction 135(1):19-27. 2008. http://www.reproduction-online.org/content/135/1/19.full.pdf+html

7.- AUTORES (p.o de firma): Velásquez JG, Canovas S, Barajas P, Marcos J, Jiménez-Movilla M, Gutiérrez Gallego R, Ballesta J, Avilés M, Coy P TITULO: Role of sialic acid in bovine sperm-zona pellucida binding. REF. REVISTA/LIBRO: Molecular Reproduction and Development 74(5):617-28. 2007.

6.- AUTORES (p.o de firma): Hoodbhoy T, Avilés M, Boris Baibakov, Olga Epifano, María Jiménez-Movilla, Lyn Gauthier, and Jurrien Dean TITULO: ZP2 and ZP3 Traffick Independently within Oocytes prior to Assembly into the Extracellular Zona Pellucida REF. REVISTA/LIBRO: Molecular and Cellular Biology 26(21):7991-7998. 2006

5.- AUTORES (p.o de firma): M. Jiménez-Movilla, M. Avilés, M.J. Gómez-Torres, P.J. Fernández-Colom, MT Castells, J. de Juan, A. Romeu, J. Ballesta. TITULO: Carbohydrate analysis of the zona pellucida and cortical granules of human oocytes by means of ultrastructural cytochemistry REF. REVISTA/LIBRO: Human Reproduction 19: 1842-1855. 2004 http://humrep.oxfordjournals.org/content/19/8/1842.full.pdf+html

4.- AUTORES (p.o de firma): M. Avilés, T. Okinaga, BD. Shur and J. Ballesta. TITULO: Differential expression of glycoside residues in the mammalian zona pellucida REF. REVISTA/LIBRO: Molecular Reproduction and Development, 57, 296-308. 2000.

3.- AUTORES (p.o. de firma): M. Avilés, M. El-Mestrah, L. Jaber, M.T. Castells, J. Ballesta and FWK Kan. TITULO: Cytochemical demonstration of modification of carbohydrates in the mouse zona pellucida during folliculogenesis. REVISTA/LIBRO: Histochemistry and Cell Biology 113: 207-219. 2000.

2.- AUTORES (p.o. de firma): M. Avilés, L. Jaber, M.T. Castells, J. Ballesta and F.W.K. Kan TITULO: Modifications of carbohydrate residues and ZP2 and ZP3 glycoproteins in the mouse zona pellucida after fertilization. REF. REVISTA/LIBRO: Biology of Reproduction 57:1155-1163. 1997 http://www.biolreprod.org/content/57/5/1155.long

1.- AUTORES (p.o. de firma): M. Avilés, I. Abascal, J.A. Martínez-Menárguez, M.T. Castells, S.R. Skalaban, J. Ballesta and J.A. Alhadeff TITULO: Immunocytochemical localization and biochemical characterization of a novel plasma membrane-associated, neutral pH optimum alpha-L-fucosidase from rat testis and epididymal spermatozoa. REF. REVISTA/LIBRO: Biochemical Journal 318:821-831. 1996 http://www.biochemj.org/bj/318/0821/3180821.pdf